Part:BBa_K541545:Experience

THE SUICIDE EXPERIMENT OF E. COLI

Our K541545 part contains IPTG promoter and the gene part of LALF protein on a backbone which has resistance to chloramphenicol. We transported this part to E. coli for testing whether E. coli would kill itself or not.

Experiments of plates with and without IPTG

Plate A1: We streaked E coli culture which has K541545 on this plate and the plate includes IPTG and Chloramphenicol.

Plate A2: We streaked E coli culture which has K541545 on this plate and the plate includes just Chloramphenicol.

Plate B1: This plate does not have IPTG and we streaked E.coli culture which does not have IPTG promoter.

PlateB2: This plate has IPTG and we streaked E coli culture which has IPTG promoter.

We prepared the B1 and B2 plates for testing toxic effect of IPTG. Because we wanted to be sure that the only factor affecting growth of E coli is LALF protein.

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